The utilization of the serum protein electrophoresis (s-PEP) in clinical chemistry is often taken for granted. This EduPath aims to trace the history and highlight the benefits of this test as it is a valuable part of modern diagnostic medicine. This test is relatively inexpensive and can be used to diagnose a number of important malignant as well as benign medical conditions. Guidance for the use of s-PEPwill be provided.